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EFFECT OF PENTACHLOROPHENOL ON SELECTED SOIL ENZYME ACTIVITIES IN A CHILEAN ANDISOL R.C. Suelo Nutr. Veg.
Diez J,M. Cristina; Gallardo A,Felipe; Saavedra,Gisselle; Cea L,Mara; Gianfreda,Liliana; Alvear Z,Marisol.
The activities of laccase, Mn-peroxidase, phosphatase and dehydrogenase were studied in a Chilean Andisol (Temuco Serie) contaminated with pentachlorophenol (PCP). Experiments were conducted on the top of 0-20 cm soil, adding different concentrations of PCP (0.41, 4.1 and 41 mg kg-1). Enzymatic activities and residual concentrations of PCP were evaluated in soil incubated at 25 ± 1 ºC for 112 days. Enzyme activities of PCP-treated soil varied according to the PCP concentration added. For instance, an inhibitory effect on Mn-peroxidase activity was not observed during incubation at the three levels of PCP, whereas acid phosphatase activity was only affected by the 0.41 mg kg-1 PCP treatment. By contrast, laccase and dehydrogenase activities were negatively...
Tipo: Journal article Palavras-chave: Pentachlorophenol; Andisol; Laccase; Mn-peroxidase; Phosphatase; Dehydrogenase.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912006000300004
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Impact of different grazing systems for bovine cattle on the soil microbiological and chemical characteristics R. Bras. Zootec.
Garcia,Martha Regina Lucizano; Sampaio,Alexandre Amstalden Moraes; Nahas,Ely.
The objective of this work was to study the effect of grazing systems: rotational, continuous and the absence of cattle, in pastures of Brachiaria brizantha on microbial activity (respiratory, potential nitrification, solubilizing, and enzymatic) and on the chemical attributes (contents of organic carbon, total nitrogen, organic phosphorus, and moisture) of Eutrustox soil over one year. Eight Canchim breed animals were used. The samples were collected in the four seasons from 2005 to 2006. The average of organic C, organic P, and moisture contents, as well as potential nitrification and solubilizing activity and urease and acid phosphatase activities were significantly higher in the spring and/or summer than in autumn and/or winter. The total nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Microbial respiratory activity; Phosphatase; Potential nitrification; Urease.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000700024
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Isolation of a thermostable acid phytase from Aspergillus niger UFV-1 with strong proteolysis resistance BJM
Monteiro,Paulo S.; Guimarães,Valéria M.; Melo,Ricardo R. de; Rezende,Sebastião T. de.
An Aspergillus niger UFV-1 phytase was characterized and made available for industrial application. The enzyme was purified via ultrafiltration followed by acid precipitation, ion exchange and gel filtration chromatography. This protein exhibited a molecular mass of 161 kDa in gel filtration and 81 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), indicating that it may be a dimer. It presented an optimum temperature of 60 °C and optimum pH of 2.0. The KM for sodium phytate hydrolysis was 30.9 mM, while the kcat and kcat/KM were 1.46 ×105 s−1 and 4.7 × 106s−1.M−1, respectively. The purified phytase exhibited broad specificity on a range of phosphorylated compounds, presenting activity on sodium phytate, p-NPP, 2-...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Phytic acid; Dephosphorylation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100251
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Lipid rafts: cell surface platforms for T cell signaling Biol. Res.
MAGEE,TONY; PIRINEN,NIINA; ADLER,JEREMY; PAGAKIS,STAMATIS N; PARMRYD,INGELA.
The Src family tyrosine kinase Lck is essential for T cell development and T cell receptor (TCR)* signaling. Lck is post-translationally fatty acylated at its N-terminus conferring membrane targeting and concentration in plasma membrane lipid rafts, which are lipid-based organisational platforms. Confocal fluorescence microscopy shows that Lck colocalises in rafts with GPI-linked proteins, the adaptor protein LAT and Ras, but not with non-raft membrane proteins including the protein tyrosine phosphatase CD45. The TCR also associates with lipid rafts and its cross-linking causes coaggregation of raft-associated proteins including Lck, but not of CD45. Cross-linking of either the TCR or rafts strongly induces specific tyrosine phosphorylation of the TCR in...
Tipo: Journal article Palavras-chave: Lipid rafts; Lck; Tyrosine kinase; T cell; Signalling; Phosphatase.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200003
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Mg 2+ -dependent ATPase activity in triatomine salivary glands (Heteroptera, Triatominae) Iheringia, Sér. Zool.
Anhê,Ana C. B. M.; Azeredo-Oliveira,Maria T. V..
ABSTRACT Mg2+-ATPase activity was detected in the three salivary glands of adult triatomines, males and females, of Triatoma infestans (Klug, 1834) and Panstrongylus megistus (Burmeister, 1835) (Heteroptera, Triatominae). A predominance of binucleated cells in D1 and D2 and mononucleated in D3 was observed, with bulky and polyploidy nuclei. ATPase activity was detected in the nuclei, possibly in euchromatin and nucleolus, where this enzyme probably acts in the transcription process. ATPase reaction was also evidenced in the nuclear membrane, which is probably associated with nuclear-cytoplasmatic transport. These characteristics indicate a high metabolism and protein synthesis, which must be essential to saliva production as well as in maintaining the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euchromatin; Gland; Nuclear membrane; Nucleolus; Phosphatase.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212017000100231
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MICROBIAL ACTIVITY OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES Rev. Microbiol.
Vieira,Francisco Cleber Sousa; Nahas,Ely.
The enzymatic activity of soil samples stored at temperatures of 5 to -12oC and at room temperature for 0-32 weeks was determined. While alkaline phosphatase and dehydrogenase activity was decreased compared to control in samples stored at low temperatures, acid phosphatase activity showed no significant change.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Phosphatase; Dehydrogenase; Soil storage.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300002
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Microbial and enzymatic activity in soil after organic composting Rev. Bras. Ciênc. Solo
Vinhal-Freitas,Isabel Cristina; Wangen,Dalcimar Regina Batista; Ferreira,Adão de Siqueira; Corrêa,Gilberto Fernandes; Wendling,Beno.
Microbial activity and biochemical properties are important indicators of the impact of organic composting on soil. The objective of this study was to evaluate some indicators of soil microbial and biochemical processes after application of compost (household waste). A Typic Acrustox, sampled at a depth of 10 cm under Cerrado biome vegetation, was evaluated in three treatments: control (soil without organic compost amendment) and soil with two doses of domestic organic compost (10 and 20 g kg-1 soil). The following properties were evaluated: released C (C-CO2): microbial respiration 15 days after incubation; microbial biomass C (MBC); total glucose (TG); metabolic quotient (qCO2); and enzyme activity of β-glucosidase and acid and alkaline phosphatase. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; Soil respiration; Microbial biomass; Glucose; Phosphatase; Β-glucosidase..
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300017
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MICROBIAL COUNTS OF DARK RED LATOSOL SAMPLES STORED AT DIFFERENT TEMPERATURES Rev. Microbiol.
Vieira,Francisco Cleber Sousa; Nahas,Ely.
The number of colony forming units (CFU) of different groups of bacteria and fungi in samples stored at temperatures of 5 to -12oC for 0-32 weeks was evaluated. The number of CFU obtained after the different periods of storage of red latossol soil was compared with the number of colonies obtained immediately after removal of soil samples (time zero). The number of total bacteria and actinomycetes in the samples remained practically unchanged throughout the storage period. The number of Gram-negative bacteria decreased by as much as 69% compared to control, while the number of Bacillus spp and of fungi increased 1.9 to 4.9 times starting from the 12th week in samples stored at 5oC. Except for the variations observed in fungal counts, the remaining groups of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Fungi; Phosphatase; Soil storage.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300001
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Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings Electron. J. Biotechnol.
Valenzuela,Eduardo; Hipp,Joseline; Alonso,Claudia; Godoy,Roberto; Alberdi,Miren; Alvarez,Maricel; Saavedra,Isabel.
At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC....
Tipo: Journal article Palavras-chave: Cellulose; Ectomycorrhizal fungi; Nothofagus; PH; Phosphatase; Temperature.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012
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Screening of filamentous fungi for production of enzymes of biotechnological interest BJM
Guimarães,Luis Henrique S.; Peixoto-Nogueira,Simone C.; Michelin,Michele; Rizzatti,Ana Carolina S.; Sandrim,Valéria C.; Zanoelo,Fabiana F; Aquino,Ana Carla M.M.; Junior,Altino B.; Polizeli,Maria de Lourdes T. M..
Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Xylanase; Glucose oxidase; Pectinase; Amylase; Rhizopus microsporus var. rhizopodiformis; Fungi.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400014
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Soil phosphorus dynamics as affected by Congo grass and P fertilizer Scientia Agricola
Rosolem,Ciro Antonio; Merlin,Alexandre; Bull,Júlio Cesar Longo.
Some plant species can change soil phosphorus (P) availability and this may be an important tool in managing tropical high fixing phosphorus soils. An experiment was conducted to evaluate phosphorus transformations in the soil and phosphatase activity during periods of Congo grass (Brachiaria ruziziensis, Germain et Evrard) growth in two tropical soils receiving 20, 40, 80, 160 mg dm-3 of inorganic P. Plants were grown for 84 days in 8-L pots. Acid phosphatase activity, P in the microbial mass, soil organic and inorganic P and P accumulation by Congo grass were evaluated. Phosphorus fertilization increased soil P availability, Congo grass yields and P accumulation in the plant. On average, less labile P forms in the soil were not changed by Congo grass;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatase; Soil fertility; Crop rotation; Brachiaria.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162014000400008
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The Study on the Localization of Acid Phosphatase Activity During Spermiogenesis in Chinese Mitten-Handed Crab, Eriocheir sinensis. OceanDocs
Liu, Guirong; Kang, Xianjiang; Guo, Mingshen; Sun, Wenjia; Wang, Qi.
Ultrastructural cytochemical techniques and electron microscopy were used for localization of acid phosphatase activity during spermiogenesis in Eriocheir sinernsis. The results showed that: Acid phosphatase was synthesized in the endoplasmic reticulum in the early spermatids. The acid phosphatase was found gradually in nucleus, the membrane of acrosomal vesicle, the cytoplasmic region and the acrosomal tubule. And then the reaction product particles became thicker during the spermiogenesis. In the mature sperm, acid phosphatase was localized in the percutor organ slightly, but it was massive and compact in the acrosomal tubule.
Tipo: Journal Contribution Palavras-chave: Phosphatase; Eriocheir sinensis.
Ano: 2007 URL: http://hdl.handle.net/1834/5838
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Weed Interference in Soybean Crop Affects Soil Microbial Activity and Biomass Planta Daninha
FIALHO,C.M.T.; SILVA,A.A.; MELO,C.A.D.; COSTA,M.D.; SOUZA,M.W.R.; REIS,L.A.C..
ABSTRACT: Weeds and microorganisms interacting with their rhizosphere may influence nutrient absorption, which is an important factor for plant competition. The purpose of this study was to evaluate the microbiological activity, inorganic phosphorus solubilization (Pi) and acid phosphatase in the cultivated soil, in a combination of soybean (Glycine max) plants and weeds. Soybeans were cultivated in monoculture and in competition with Bidens pilosa, Brachiaria decumbens (Syn. Urochloa decumbens) and Eleusine indica, under two conditions: a) plants competing without contact between the roots b) plants competing with contact between the roots. A nylon screen with a 50 µm mesh was added to prevent contact between the roots of the species in competition so...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Soil respiratory rate; Metabolic quotient; Inorganic phosphorus solubilization; Phosphatase.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100247
Registros recuperados: 13
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